Label-free differentiation of functional zones in mature mouse placenta using micro-Raman imaging

Name: Label-free differentiation of functional zones in mature mouse placenta using micro-Raman imaging
Author: Celik-Ozenci, C., Unlu, Mehmet Burcin, Ucak, M., Durkut, B., Parlatan, U., Kecoglu, I., Bektas, N. İ., Inanc, A.

İsim	Label-free differentiation of functional zones in mature mouse placenta using micro-Raman imaging
Yazar	Celik-Ozenci, C., Unlu, Mehmet Burcin, Ucak, M., Durkut, B., Parlatan, U., Kecoglu, I., Bektas, N. İ., Inanc, A.
Basım Tarihi:	2024-05-01
Basım Yeri	- Optica Publishing
Tür	Süreli Yayın
Dil	İngilizce
Dijital	Evet
Yazma	Hayır
Kütüphane:	Özyeğin Üniversitesi
Demirbaş Numarası	2156-7085
Kayıt Numarası	e509718c-e1f7-4a89-863c-228586788f94
Lokasyon	Natural and Mathematical Sciences
Tarih	2024-05-01
Notlar	Directorate of Presidential Strategy and Budget of Turkey ; TÜBİTAK ; Akdeniz University
Örnek Metin	In histopathology, it is highly crucial to have chemical and structural information about tissues. Additionally, the segmentation of zones within a tissue plays a vital role in investigating the functions of these regions for better diagnosis and treatment. The placenta plays a vital role in embryonic and fetal development and in diagnosing some diseases associated with its dysfunction. This study provides a label -free approach to obtain the images of mature mouse placenta together with the chemical differences between the tissue compartments using Raman spectroscopy. To generate the Raman images, spectra of placental tissue were collected using a custom-built optical setup. The pre-processed spectra were analyzed using statistical and machine learning methods to acquire the Raman maps. We found that the placental regions called decidua and the labyrinth zone are biochemically distinct from the junctional zone. A histologist performed a comparison and evaluation of the Raman map with histological images of the placental tissue, and they were found to agree. The results of this study show that Raman spectroscopy offers the possibility of label -free monitoring of the placental tissue from mature mice while simultaneously revealing crucial structural information about the zones.
DOI	10.1364/BOE.521500
Cilt	15

Kaynağa git Özyeğin Üniversitesi

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Özyeğin Üniversitesi

Kaynağa git

Label-free differentiation of functional zones in mature mouse placenta using micro-Raman imaging

Yazar Celik-Ozenci, C., Unlu, Mehmet Burcin, Ucak, M., Durkut, B., Parlatan, U., Kecoglu, I., Bektas, N. İ., Inanc, A.

Basım Tarihi 2024-05-01

Basım Yeri - Optica Publishing

Tür Süreli Yayın

Dil İngilizce

Dijital Evet

Yazma Hayır

Kütüphane Özyeğin Üniversitesi

Demirbaş Numarası 2156-7085

Kayıt Numarası e509718c-e1f7-4a89-863c-228586788f94

Lokasyon Natural and Mathematical Sciences

Tarih 2024-05-01

Notlar Directorate of Presidential Strategy and Budget of Turkey ; TÜBİTAK ; Akdeniz University

Örnek Metin In histopathology, it is highly crucial to have chemical and structural information about tissues. Additionally, the segmentation of zones within a tissue plays a vital role in investigating the functions of these regions for better diagnosis and treatment. The placenta plays a vital role in embryonic and fetal development and in diagnosing some diseases associated with its dysfunction. This study provides a label -free approach to obtain the images of mature mouse placenta together with the chemical differences between the tissue compartments using Raman spectroscopy. To generate the Raman images, spectra of placental tissue were collected using a custom-built optical setup. The pre-processed spectra were analyzed using statistical and machine learning methods to acquire the Raman maps. We found that the placental regions called decidua and the labyrinth zone are biochemically distinct from the junctional zone. A histologist performed a comparison and evaluation of the Raman map with histological images of the placental tissue, and they were found to agree. The results of this study show that Raman spectroscopy offers the possibility of label -free monitoring of the placental tissue from mature mice while simultaneously revealing crucial structural information about the zones.

DOI 10.1364/BOE.521500

Cilt 15