Author
Celik-Ozenci, C., Unlu, Mehmet Burcin, Ucak, M., Durkut, B., Parlatan, U., Kecoglu, I., Bektas, N. İ., Inanc, A.
Publication Date
2024-05-01
Publication Place
-
Optica Publishing
Type
Periodical
Language
English
Digital
Yes
Manuscript
No
Library
Özyeğin University
Library Asset ID
2156-7085
Record ID
e509718c-e1f7-4a89-863c-228586788f94
Library Location
Natural and Mathematical Sciences
Date
2024-05-01
Notes
Directorate of Presidential Strategy and Budget of Turkey ; TÜBİTAK ; Akdeniz University
Sample Text
In histopathology, it is highly crucial to have chemical and structural information about tissues. Additionally, the segmentation of zones within a tissue plays a vital role in investigating the functions of these regions for better diagnosis and treatment. The placenta plays a vital role in embryonic and fetal development and in diagnosing some diseases associated with its dysfunction. This study provides a label -free approach to obtain the images of mature mouse placenta together with the chemical differences between the tissue compartments using Raman spectroscopy. To generate the Raman images, spectra of placental tissue were collected using a custom-built optical setup. The pre-processed spectra were analyzed using statistical and machine learning methods to acquire the Raman maps. We found that the placental regions called decidua and the labyrinth zone are biochemically distinct from the junctional zone. A histologist performed a comparison and evaluation of the Raman map with histological images of the placental tissue, and they were found to agree. The results of this study show that Raman spectroscopy offers the possibility of label -free monitoring of the placental tissue from mature mice while simultaneously revealing crucial structural information about the zones.
DOI
10.1364/BOE.521500
Cilt
15
