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Design and simulation of microfluidic device for metabolite screening and quantitative monitoring of drug uptake in cancer cells

Title	Design and simulation of microfluidic device for metabolite screening and quantitative monitoring of drug uptake in cancer cells
Author	Asif, Afia, Khawaldeh, S., Khan, Muhammad Salman, Tekin, Ahmet
Publication Date:	2018
Publication Place	- Sciendo
Subject	Microfluidics, Micro and nano fabrication, Photolithography, Drug uptake, Cancer cells, Multiphysics simulation, In vitro, Metabolite screening
Type	Periodical
Language	English
Digital	Yes
Manuscript	No
Library:	Özyeğin University
Library Asset ID	18915469
Record ID	440c095f-8e93-4ba2-b1ac-f443f1bb5a15
Library Location	Electrical & Electronics Engineering
Date	2018
Sample Text	Although liquid-liquid extraction methods are currently being applied in many areas such as analytical chemistry, biochemical engineering, biochemistry, and biological applications, accessibility and usability of microfluidics in practical daily life fields are still bounded. Suspended microfluidic devices have the potential to lessen the obstacles, but the absence of robust design rules have hampered their usage. The primary objective of this work is to design and fabricate a microfluidic device to quantitatively monitor the drug uptake of cancer cells. Liquid-liquid extraction is used to quantify the drug uptake. In this research work, designs and simulations of two different microfluidic devices for carrying out multiplex solution experiments are proposed to test their efficiency. These simplified miniaturized chips would serve as suspended microfluidic metabolites extraction platform as it allows extracting the metabolites produced from the cancer cells as a result of applying a specific drug type for a certain period of time. These devices would be fabricated by making polydimethylsiloxane (PDMS) molds from the negative master mold using soft lithography. Furthermore, it can leverage to provide versatile functionalities like high throughput screening, cancer cell invasions, protein purification, and small molecules extractions. As per previous studies, PDMS has been depicting better stability with various solvents and has proved to be a reliable and cost effective material to be used for fabrication, though the sensitivity of the chip would be analyzed by cross contamination and of solvents within the channels of device.
DOI	10.2478/joeb-2018-0003
Cilt	9

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Özyeğin University

Design and simulation of microfluidic device for metabolite screening and quantitative monitoring of drug uptake in cancer cells

Author Asif, Afia, Khawaldeh, S., Khan, Muhammad Salman, Tekin, Ahmet

Publication Date 2018

Publication Place - Sciendo

Subject Microfluidics, Micro and nano fabrication, Photolithography, Drug uptake, Cancer cells, Multiphysics simulation, In vitro, Metabolite screening

Type Periodical

Language English

Digital Yes

Manuscript No

Library Özyeğin University

Library Asset ID 18915469

Record ID 440c095f-8e93-4ba2-b1ac-f443f1bb5a15

Library Location Electrical & Electronics Engineering

Date 2018

Sample Text Although liquid-liquid extraction methods are currently being applied in many areas such as analytical chemistry, biochemical engineering, biochemistry, and biological applications, accessibility and usability of microfluidics in practical daily life fields are still bounded. Suspended microfluidic devices have the potential to lessen the obstacles, but the absence of robust design rules have hampered their usage. The primary objective of this work is to design and fabricate a microfluidic device to quantitatively monitor the drug uptake of cancer cells. Liquid-liquid extraction is used to quantify the drug uptake. In this research work, designs and simulations of two different microfluidic devices for carrying out multiplex solution experiments are proposed to test their efficiency. These simplified miniaturized chips would serve as suspended microfluidic metabolites extraction platform as it allows extracting the metabolites produced from the cancer cells as a result of applying a specific drug type for a certain period of time. These devices would be fabricated by making polydimethylsiloxane (PDMS) molds from the negative master mold using soft lithography. Furthermore, it can leverage to provide versatile functionalities like high throughput screening, cancer cell invasions, protein purification, and small molecules extractions. As per previous studies, PDMS has been depicting better stability with various solvents and has proved to be a reliable and cost effective material to be used for fabrication, though the sensitivity of the chip would be analyzed by cross contamination and of solvents within the channels of device.

DOI 10.2478/joeb-2018-0003

Cilt 9