Author
Wang, S., Tasoglu, S., Chen, P. Z., Chen, M., Akbaş, Ragıp, Wach, S., Ozdemir, C. İ., Gurkan, U. A., Giguel, F. F., Kuritzkes, D. R., Demirci, U.
Publication Date
2014-01-22
Publication Place
-
Nature
Subject
Translational research, Laboratory techniques and procedures
Type
Periodical
Language
English
Digital
Yes
Manuscript
No
Library
Özyeğin University
Library Asset ID
2045-2322
Record ID
fd0a4ae5-f1b7-4d5c-b1d1-fe6748d651c9
Library Location
Civil Engineering
Date
2014-01-22
Notes
the Center for Integration of Medicine and Innovative Technology ; the U.S. Army Medical Research & Materiel Command (USAMRMC) ; the Telemedicine & Advanced Technology Research Center (TATRC).
Sample Text
HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via “moving the substrate”, as opposed to “flowing liquid” in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays.
DOI
10.1038/srep03796
Cilt
4
