A novel microfluidic-based fluorescence detection method reveals heavy atom effects on photophysics of fluorophores with high triplet quantum yield: A numerical simulation study

Name: A novel microfluidic-based fluorescence detection method reveals heavy atom effects on photophysics of fluorophores with high triplet quantum yield: A numerical simulation study
Author: Dirican, Selim Can, Demirbay, Barış

İsim	A novel microfluidic-based fluorescence detection method reveals heavy atom effects on photophysics of fluorophores with high triplet quantum yield: A numerical simulation study
Yazar	Dirican, Selim Can, Demirbay, Barış
Basım Tarihi:	2025-01-20
Konu	Fluorescence spectroscopy, Fluorophore blinking, Heavy atom effect, Microfluidics, Photophysics, Widefield microscopy
Tür	Süreli Yayın
Dil	İngilizce
Dijital	Evet
Yazma	Hayır
Kütüphane:	Özyeğin Üniversitesi
Demirbaş Numarası	1522-7243
Kayıt Numarası	55661ab4-e671-4514-aaa7-51ffe57ff261
Lokasyon	Natural and Mathematical Sciences
Tarih	2025-01-20
Örnek Metin	The present study introduces the idea of a novel fluorescence-based imaging technique combined with a microfluidic platform that enables a precise control of dark transient state populations of fluorescent probes flowing over a uniform, top flat supergaussian excitation field with a constant flow rate. To demonstrate the imaging capability of the proposed detection method, numerical simulations have been performed by considering laser, microscope and flow parameters of experimental setup together with photophysical model and electronic transition rates of fluorescent dyes. As an output data to be assessed, fluorescence image data is simulated numerically for bromine-free carboxyfluorescein and its brominated derivatives having different numbers of bromine atoms. Based on the magnitudes of applied excitation irradiances and flow rates, which can be manually controlled by user during experiments, the presence of dark state populations can appear as broadening, shifts and decays in normalized fluorescence intensity signals that are computed from simulated fluorescence images. As such changes in signals become more pronounced upon an increase in the degree of bromination, it is elicited that heavy atom effect can be resolved by properly tuning excitation powers of laser and flow rates. Proposed imaging method has potential to provide invaluable means to conventional fluorescence methods and can open up new perspectives in biomedical research.
DOI	10.1002/bio.70090
Cilt	40

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A novel microfluidic-based fluorescence detection method reveals heavy atom effects on photophysics of fluorophores with high triplet quantum yield: A numerical simulation study

Yazar Dirican, Selim Can, Demirbay, Barış

Basım Tarihi 2025-01-20

Konu Fluorescence spectroscopy, Fluorophore blinking, Heavy atom effect, Microfluidics, Photophysics, Widefield microscopy

Tür Süreli Yayın

Dil İngilizce

Dijital Evet

Yazma Hayır

Kütüphane Özyeğin Üniversitesi

Demirbaş Numarası 1522-7243

Kayıt Numarası 55661ab4-e671-4514-aaa7-51ffe57ff261

Lokasyon Natural and Mathematical Sciences

Tarih 2025-01-20

Örnek Metin The present study introduces the idea of a novel fluorescence-based imaging technique combined with a microfluidic platform that enables a precise control of dark transient state populations of fluorescent probes flowing over a uniform, top flat supergaussian excitation field with a constant flow rate. To demonstrate the imaging capability of the proposed detection method, numerical simulations have been performed by considering laser, microscope and flow parameters of experimental setup together with photophysical model and electronic transition rates of fluorescent dyes. As an output data to be assessed, fluorescence image data is simulated numerically for bromine-free carboxyfluorescein and its brominated derivatives having different numbers of bromine atoms. Based on the magnitudes of applied excitation irradiances and flow rates, which can be manually controlled by user during experiments, the presence of dark state populations can appear as broadening, shifts and decays in normalized fluorescence intensity signals that are computed from simulated fluorescence images. As such changes in signals become more pronounced upon an increase in the degree of bromination, it is elicited that heavy atom effect can be resolved by properly tuning excitation powers of laser and flow rates. Proposed imaging method has potential to provide invaluable means to conventional fluorescence methods and can open up new perspectives in biomedical research.

DOI 10.1002/bio.70090

Cilt 40