Micro-a-fluidics ELISA for rapid CD4 cell count at the point-of-care

Name: Micro-a-fluidics ELISA for rapid CD4 cell count at the point-of-care
Author: Wang, S., Tasoglu, S., Chen, P. Z., Chen, M., Akbaş, Ragıp, Wach, S., Ozdemir, C. İ., Gurkan, U. A., Giguel, F. F., Kuritzkes, D. R., Demirci, U.

İsim	Micro-a-fluidics ELISA for rapid CD4 cell count at the point-of-care
Yazar	Wang, S., Tasoglu, S., Chen, P. Z., Chen, M., Akbaş, Ragıp, Wach, S., Ozdemir, C. İ., Gurkan, U. A., Giguel, F. F., Kuritzkes, D. R., Demirci, U.
Basım Tarihi:	2014-01-22
Basım Yeri	- Nature
Konu	Translational research, Laboratory techniques and procedures
Tür	Süreli Yayın
Dil	İngilizce
Dijital	Evet
Yazma	Hayır
Kütüphane:	Özyeğin Üniversitesi
Demirbaş Numarası	2045-2322
Kayıt Numarası	fd0a4ae5-f1b7-4d5c-b1d1-fe6748d651c9
Lokasyon	Civil Engineering
Tarih	2014-01-22
Notlar	the Center for Integration of Medicine and Innovative Technology ; the U.S. Army Medical Research & Materiel Command (USAMRMC) ; the Telemedicine & Advanced Technology Research Center (TATRC).
Örnek Metin	HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via “moving the substrate”, as opposed to “flowing liquid” in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays.
DOI	10.1038/srep03796
Cilt	4

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Micro-a-fluidics ELISA for rapid CD4 cell count at the point-of-care

Yazar Wang, S., Tasoglu, S., Chen, P. Z., Chen, M., Akbaş, Ragıp, Wach, S., Ozdemir, C. İ., Gurkan, U. A., Giguel, F. F., Kuritzkes, D. R., Demirci, U.

Basım Tarihi 2014-01-22

Basım Yeri - Nature

Konu Translational research, Laboratory techniques and procedures

Tür Süreli Yayın

Dil İngilizce

Dijital Evet

Yazma Hayır

Kütüphane Özyeğin Üniversitesi

Demirbaş Numarası 2045-2322

Kayıt Numarası fd0a4ae5-f1b7-4d5c-b1d1-fe6748d651c9

Lokasyon Civil Engineering

Tarih 2014-01-22

Notlar the Center for Integration of Medicine and Innovative Technology ; the U.S. Army Medical Research & Materiel Command (USAMRMC) ; the Telemedicine & Advanced Technology Research Center (TATRC).

Örnek Metin HIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via “moving the substrate”, as opposed to “flowing liquid” in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays.

DOI 10.1038/srep03796

Cilt 4