Microchip ELISA coupled with cell phone to detect ovarian cancer HE4 biomarker in urine

Name: Microchip ELISA coupled with cell phone to detect ovarian cancer HE4 biomarker in urine
Author: Wang, S., Akbaş, Ragıp, Demirci, U.

İsim	Microchip ELISA coupled with cell phone to detect ovarian cancer HE4 biomarker in urine
Yazar	Wang, S., Akbaş, Ragıp, Demirci, U.
Basım Tarihi:	2014
Basım Yeri	- Springer Science+Business Media
Konu	Microchip ELISA, Mobile application, Diagnostics, HE4 biomarker
Tür	Kitap
Dil	İngilizce
Dijital	Evet
Yazma	Hayır
Kütüphane:	Özyeğin Üniversitesi
Demirbaş Numarası	978-1-4939-2172-0
Kayıt Numarası	efb2e6eb-6b53-44c2-b3f2-0036bf7ebd51
Lokasyon	Civil Engineering
Tarih	2014
Örnek Metin	Ovarian cancer is a leading cause of death from gynecologic cancers in the USA, and early diagnosis can potentially increase 5-year survival rate. Detection of biomarkers derived from hyperplasia of epithelial tissue by enzyme-linked immunosorbent assay (ELISA) proves to be a practical way of early diagnosis of ovarian cancer. However, ELISA is commonly performed in a laboratory setting, and it cannot be used in a clinical setting for on-site consultation. We have shown a microchip ELISA that detects HE4, an ovarian cancer biomarker, from urine using a cell phone integrated with a mobile application for imaging and data analysis. In microchip ELISA, HE4 from urine was first absorbed on the surface; the primary and secondary antibodies were subsequently anchored on the surface via immuno-reaction; and addition of substrate led to color development because of enzymatic labeling. The microchip after color development was imaged using a cell phone, and the color intensity was analyzed by an integrated mobile application. By comparing with an ELISA standard curve, the concentration of HE4 was reported on the cell phone screen. The presented microchip ELISA coupled with a cell phone is portable as opposed to traditional ELISA, and this method can facilitate the detection of ovarian cancer at the point-of-care (POC).
DOI	10.1007/978-1-4939-2172-0_8
Cilt	1256

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Microchip ELISA coupled with cell phone to detect ovarian cancer HE4 biomarker in urine

Yazar Wang, S., Akbaş, Ragıp, Demirci, U.

Basım Tarihi 2014

Basım Yeri - Springer Science+Business Media

Konu Microchip ELISA, Mobile application, Diagnostics, HE4 biomarker

Tür Kitap

Dil İngilizce

Dijital Evet

Yazma Hayır

Kütüphane Özyeğin Üniversitesi

Demirbaş Numarası 978-1-4939-2172-0

Kayıt Numarası efb2e6eb-6b53-44c2-b3f2-0036bf7ebd51

Lokasyon Civil Engineering

Tarih 2014

Örnek Metin Ovarian cancer is a leading cause of death from gynecologic cancers in the USA, and early diagnosis can potentially increase 5-year survival rate. Detection of biomarkers derived from hyperplasia of epithelial tissue by enzyme-linked immunosorbent assay (ELISA) proves to be a practical way of early diagnosis of ovarian cancer. However, ELISA is commonly performed in a laboratory setting, and it cannot be used in a clinical setting for on-site consultation. We have shown a microchip ELISA that detects HE4, an ovarian cancer biomarker, from urine using a cell phone integrated with a mobile application for imaging and data analysis. In microchip ELISA, HE4 from urine was first absorbed on the surface; the primary and secondary antibodies were subsequently anchored on the surface via immuno-reaction; and addition of substrate led to color development because of enzymatic labeling. The microchip after color development was imaged using a cell phone, and the color intensity was analyzed by an integrated mobile application. By comparing with an ELISA standard curve, the concentration of HE4 was reported on the cell phone screen. The presented microchip ELISA coupled with a cell phone is portable as opposed to traditional ELISA, and this method can facilitate the detection of ovarian cancer at the point-of-care (POC).

DOI 10.1007/978-1-4939-2172-0_8

Cilt 1256